Chiral separation of thiazide diuretics by HPLC on Chiralcel OD-RHR, Chiralcel OJ-RR and Chirobiotic-TkR phases

Bala´zs Visegra´dy^a, Tu¨nde Konecsni ^a, Nina Grobuschek^b, Martin G. Schmid ^b, Ferenc Kila´r^a, Hassan Y. Aboul-Enein^c, Gerald Gu¨bitz^b,*

^aCentral Research Laboratory, Faculty of Medicine, University of Pe´cs, Szigeti u´ t 12, H-7643 Pe´cs, Hungary

^bInstitute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University Graz, Universita¨tsplatz 1, A-8010 Graz, Austria

^cBioanalytical and Drug Development Laboratory, Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia

Abstract

This contribution deals with comparative studies on the chiral separation of thiazide diuretics using cellulose tris(3,5-dimethylphenylcarbamate) (Chiralcel OD-RHR), cellulose tris(4-methylbenzoate) (Chiralcel OJ-RR) and teicoplanin (Chirobiotic TkR) phases. All columns showed good chiral recognition ability for this class of compounds. Out of seven compounds investigated, six were resolved with baseline resolution with at least one of the three columns.

Keywords: Enantiomer separation; Teicoplanin chiral stationary phase; Cellulose-based stationary phase;Diuretics

Sumber : J. Biochem. Biophys. Methods 53 (2002) 15–24

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