Enantioseparation of Some Clinically Used Drugs by Capillary Electrophoresis Using Sulfated b-Cyclodextrin as a Chiral Selector

G. S. Yang¹, D. M. Chen², Y. Yang³, B. Tang², J. J. Gao², H. Y. Aboul-Enein⁴,&, B. Koppenhoefer⁵

1 Department of Chemistry, Shandong University, Jinan 250100, P. R. China

2 Shandong Key Laboratory of Chemical Function Materials, Shandong Normal University, Jinan 250014, P.R. China

3 Cell Biology Laboratory, School of Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250014, P. R. China

4 Centre for Clinical Research (MBC 03-65), King Faisal Specialist Hospital and Research Centre, P.O. Box 3354 Riyadh 11211, Saudi Arabia;

E-Mail: enein@kfshrc.edu.sa

5 Institute of Organic Chemistry, University of Tu¨bingen, Auf der Morgenstelle 18, D- 72076, Tu¨bingen, Germany

Abstract

The enantiomeric separation of 37 clinically used racemic basic drugs among 50 drugs was achieved using sulfated b-cyclodextrin (S-b-CD) as chiral selector at pH2.5 and in the reversed polarity mode. The results obtained in this study were different from the one obtained using neutral b-CD and its derivatives as chiral selectors. Using S-b-CD as chiral selector did not require the presence of the substructure 4H to achieve chiral separation as observed with b-Cyclodextrin (b-CD) and its derivatives since among the 37 separated drugs only 7 possess the 4H substructure. The chiral discrimination depends on the appropriate interaction between the analyte and the sulfated b-cyclodextrin.

Keywords : Capillary electrophoresis, Chiral separation, Sulfated b-cyclodextrin sodium salt, Racemic basic drugs, Reversed polarity model

Sumber : Chromatographia 2005, 62, October (No. 7/8)

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